Practical aerobic membrane filtration blood culture technique: clinical blood culture trial
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چکیده
منابع مشابه
Practical aerobic membrane filtration blood culture technique: clinical blood culture trial.
During the course of preliminary clinical trials of an improved membrane filter blood culture system, filter plugging produced by a gelling of the lysing solution was observed when the patients had high leukocyte counts. A solution of streptokinase-streptodornase (Varidase, Lederle Laboratories) dissolved or prevented the gel and permitted rapid filtration without plugging. With streptokinase-s...
متن کاملPractical aerobic membrane filtration blood culture technique: development of procedure.
The advantages of a membrane filter system for blood culturing have been realized for many years. Lysing of the blood prior to filtration is a convenient way to proceed, but previously described lysing procedures result in loss of certain organisms, particularly gram-negative bacilli. Four concentrations of Triton X-100 and sodium carbonate were studied in vitro, and their lysing and antibacter...
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A blood culture system was developed in which a lysing agent (either Tween 20, one of several other polyoxyethylene adducts, digitonin, or Triton X-100) is added to the blood culture medium. Of 33 Triton compounds, 9 lysed human blood, as did 7 of 21 polyoxyethylene compounds and digitonin, all at a concentration of 0.05%. Under the specific test conditions, three of the hemolytic polyoxyethyle...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1975
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.1.1.37-43.1975